Slovenia has introduced new border restrictions for migrants as part of efforts to close the Balkans route from Greece to Western Europe.
Only migrants who plan to seek asylum in the country, or those with clear humanitarian needs will be allowed entry.
In reaction, Serbia said it would close its borders with Macedonia and Bulgaria to those without valid documents.
The future of the EU's passport-free Schengen zone is already in doubt, reports BBC.
Eight of its members, including Austria, Hungary and Slovakia, have tightened border controls, leaving thousands of migrants stranded in Greece.
Europe is facing its biggest refugee crisis since World War Two. Last year, more than a million people entered the EU illegally by boat. Most of them were Syrian, fleeing the country's civil war.
Slovenia, which is a EU member, has been used as a transit country by migrants trying to reach Germany and other northern European states.
But Slovenian Prime Minister Miro Cerar said on Tuesday the Balkan route was now effectively "shutting down".
He said the restrictions, which came into force at midnight local time (2300 GMT), were part of a wider initiative which would see other Balkan countries, as well as Greece and with the cooperation of Turkey, turn back "all irregular migrants".
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